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Alternative screening method for positive pLKO-Clones
Referring to the Addgene’s cloning protocol for the lentiviral vector pLKO.1 I would like to propose an alternative screening method für positive clones. read more »
Busy Days
The last days were filled with bustling activity and brought big changes. two weeks ago my new mentor Peter and i went to the clinic for tumor biology in freiburg to learn a new technique called “spheroid-assay”. this assay can be used to analyse the three-dimensional groth of new blood vessels in a cell culture. we spent the whole day peeking into culture plates, medium flasks and microscopes, thorougly writing down everything. the guys there were great (esp. big thank you to holger and oliver)! So, in the next few months Peter and myself will establish the spheroid-assay at our lab at the pancreas research center in heidelberg.
last week i got a phone call from the surgery’s residency director asking me to begin my clinical work next monday (!) initially i was supposed to start my residency in april… just the other day i gave the first “release candidate” of my medical thesis to dr. schwartz-albiez, who is my supervisor at the dkfz. i keep my fingers crossed that he agrees on submitting the work without further editing. yipee! today i got my medical identity card. now i am officially enabled to get all those fine drugs and pills (just for my own requirements) at the pharmacy
