doktor-schubert · net http://en.doktor-schubert.net Homepage of Dr. med. Mario Schubert Sun, 18 May 2008 14:19:39 +0000 http://wordpress.org/?v=2.6 en Breast-Cancer-Vaccination Helps with Survival! http://en.doktor-schubert.net/2008/breast-cancer-vaccination-helps-with-survival/ http://en.doktor-schubert.net/2008/breast-cancer-vaccination-helps-with-survival/#comments Fri, 18 Apr 2008 15:52:53 +0000 Mario http://doktor-schubert.net/en/?p=77 neuvax.jpgVaccination against the tumor feature HER2/neu could improve the survival rate of patients with breast cancer, according to the preliminary conclusion of a study conducted by Linda Benavides and their employees from San Antonio, Texas. The American scientist now presented the results of their studies with the experimental vaccine NeuVax (E75), in which a total of 163 patients participated, at the annual meeting of the American Association for Cancer Research[1].
Especially the group of patients in which only a low expression of HER2 is present in the tumor (”low expressors”), seems to profit from the vaccination. After succesful first line treatment of breast cancer with conventional chemotherapy a relapse of the disease could be greatly reduced through vaccination - only 8% of the vaccinated patients got a relapse, while the recurrence rate in the non-vaccinated patients was 21%. The vaccine appears to have a positive effect on the overall survival as well; while all vaccinated patients from the group of “low expressors” are still live, unfortunately 20% of patients in the control group succumbed to to their illness.
Interestingly, especially patients for whom treatment with the monoclonal HER2/neu-Antibody Mabthera is not applicable, might benefit from this new form of therapy.

Due to the small numbers of patients in this preliminary study there are still no definitive conclusions. We may be very excited about the results of the impact studies, on which about 700 to 1000 patients will participate.

(semi-automated translation thanks to google.com)

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Literature & Sources

  1. Medical News Today: low HER2 Expressing Breast Cancer Patients Respond To Preferentially Apthera’s NeuVax (16.04.2008)
]]> http://en.doktor-schubert.net/2008/breast-cancer-vaccination-helps-with-survival/feed/ I Got Awarded as Best Young Scientist http://en.doktor-schubert.net/2008/award-as-best-young-scientist/ http://en.doktor-schubert.net/2008/award-as-best-young-scientist/#comments Wed, 05 Mar 2008 12:26:10 +0000 Mario http://doktor-schubert.net/2008/auszeichnung-als-bester-nachwuchsautor/ The German Society for Internal Medicine (DGIM) has awarded me as best author under 35 years in the field of hematology/oncology on their annual meeting. I was honoured for my publication:

Die N-Cadherin / [beta]-Catenin Interaktion spielt eine wichtige Rolle bei Selbsterhalt und Differenzierung humaner hämatopoetischer Stammzellen.

The honour and my gratitude goes to my co-authors, especially Larissa Pietsch.

As one out of 10 “best young authors” I am electible to the Young Investigators Award 2008. The election process requires a a 15min plenary talk on April, 01 2008 at the Rhein-Main-Halls at Wiesbaden. The prestigeous price is endowed with 3000 Euro.

Further Information

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]]> http://en.doktor-schubert.net/2008/award-as-best-young-scientist/feed/ Bla-bla Blubb (My Visit to the Georgia-Aquarium) http://en.doktor-schubert.net/2007/bla-bla-blubb/ http://en.doktor-schubert.net/2007/bla-bla-blubb/#comments Fri, 28 Dec 2007 06:12:48 +0000 Mario http://doktor-schubert.de/?p=76&lang=de During my stay in Atlanta for the ASH-Annual Meeting I had the opportunity to visit the Georgia-Aquarium - one of the biggest Aquaria worldwide. In five wonderful “theme worlds” different natural maritime habitats and their inhabitants were presented.

Pictures from the Georgia Aquarium

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]]> http://en.doktor-schubert.net/2007/bla-bla-blubb/feed/ ASH-Meeting 2007 in Atlanta http://en.doktor-schubert.net/2007/ash-2007-atlanta/ http://en.doktor-schubert.net/2007/ash-2007-atlanta/#comments Thu, 27 Dec 2007 05:04:52 +0000 Mario http://doktor-schubert.de/?p=75&lang=de Every year the American Society of Hematology (ASH) invites to their meeting that lasts several days. This month I had the opportunity to attend to this biggest meeting for hematology/oncology worldwide. The Event took place in Atlanta, the capital of Georgia.

Pictures from the ASH-Meeting in Atlanta

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]]> http://en.doktor-schubert.net/2007/ash-2007-atlanta/feed/ Alternative screening method for positive pLKO-Clones http://en.doktor-schubert.net/2007/alternative-plko-screening-method/ http://en.doktor-schubert.net/2007/alternative-plko-screening-method/#comments Wed, 26 Sep 2007 22:20:36 +0000 Mario http://www.doktor-schubert.net/uncategorized/alternative-screeningmethode-fuer-plko/ Digest of pLKO with XhoIReferring to the Addgene’s cloning protocol for the lentiviral vector pLKO.1 I would like to propose an alternative screening method für positive clones.
The original protocol suggested a double digest with EcoRI and NcoI that resulted in two relatively large bands - one at 2kb and one at 5kb. In concequence, the length relations made it hard to reliably identify clones with an intact isert. With this alternative Protocol, conducting a digestion with XhoI, you get a gel picture that is, in my opinion, much easier to interpret.

unfortunately, the protocol is currently only available in German.

Alternatives Screening-Protokoll

Material

  • Plasmid Miniprep Kit (Qiagen #27104)
  • XhoI (Fermentas #ER0691)
  • TopVision LE Agarose (Fermentas #R0491)
  • Rotiphorese 10x TBE-Buffer (Carl Roth 3061.1)

Procedere

  1. (Tag 1) Nach der Trafo und 16-18h Kultur auf LB-Amp Agarplatten ca. 5-10 Kolonien von der Platte picken und in 3ml LB-Amp übernacht kultivieren.
  2. (Tag 2) 2ml der ÜN-Kultur für eine Mini-Präp verwenden,
    Achtung: für den Elutionsschritt ddH2O oder Tris-HCL Puffer nehmen, da EDTA die anschließenden enzymatischen Reaktionen stören kann. Die restliche Kultur wird vorübergehend im Kühlschrank bei +4°C gelagert oder kann als Glycerolstock eingefroren werden.
  3. Für die photometrische Messung des DNA-Gehalts, mache eine 1:100 Verdünnung mit 5µl miniprep DNA und 495µl ddH2O
  4. Setze einen Master-Mix für den Verdau-Ansatz an (plane für n+2 Ansätze).
    Berechne für jeden Ansatz:

    • 1µg miniprep DNA
    • 2µl Puffer R (Fermentas 10x Puffer für XhoI)
    • 0.5µl XhoI
    • auf 20µl ddH2O
  5. Inkubiere die Verdauansätze bei 37°C für 1-2h
  6. Trage die Verdauansätze auf ein 1% Agarosegel auf und mache einen Gellauf bei 10V/cm für ca. 45 Minuten.

Positive Klone haben eine deutliche Bande bei 380bp, außerdem eine schwache Bande bei 190bp und den “Rest” bei ~5000bp. Diese Klone sollten zur Sequenzierung eingeschickt werden. Bei nicht intakten Inserts ergibt sich ein Bandenshift von 50-80bp. Klone mit religiertem Stuffer haben außerdem eine Bande bei ~2000bp.

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]]> http://en.doktor-schubert.net/2007/alternative-plko-screening-method/feed/ Used Plugins http://en.doktor-schubert.net/2007/used-plugins/ http://en.doktor-schubert.net/2007/used-plugins/#comments Mon, 20 Aug 2007 19:38:47 +0000 Mario http://www.doktor-schubert.net/uncategorized/verwendete-plugins/ Derzeit sind 18 Plugins installiert: 17 aktive Plugins und 1 inaktive Plugins.

Active Plugins

  • Akismet 2.1.5
    Akismet checks your comments against the Akismet web service to see if they look like spam or not. You need a WordPress.com API key to use it. You can review the spam it catches under “Comments.” To show off your Akismet stats just put <?php akismet_counter(); ?> in your template. See also: WP Stats plugin.
    [ Autor: Matt Mullenweg ] [ url ]
  • Drain Hole 2.0.18
    A download management and monitoring plugin with statistics and file protection
    [ Autor: John Godley ] [ url ]
  • Google XML Sitemaps 3.1
    This plugin will generate a sitemaps.org compatible sitemap of your WordPress blog which is supported by Ask.com, Google, MSN Search and YAHOO. Configuration Page
    [ Autor: Arne Brachhold ] [ url ]
  • Lighter Menus 2.7
    Creates Drop Down Menus for WordPress Admin Panels. Fast to load, adaptable to color schemes, comes with silk icons, a option page, and a design that fits within the Wordpress 2.5 interface taking the less room possible.
    [ Autor: corpodibacco ] [ url ]
  • Lightview Plus 1.1.10
    Used to overlay images on the webpage and to automatically add links to images. Lightview by Nick Stakenburg. Read installation instructions on my website. Since version 1.1.10 lightview-plus is delivered without lightview-javascript. Please read the installation instructions on my website
    [ Autor: Puzich ] [ url ]
  • Link Indication 3.2
    Adds CSS class attributes to external links and optionally to any other link types such as flickr, wikipedia, imdb, etc.
    [ Autor: Michael Woehrer ] [ url ]
  • Share This 1.4
    Let your visitors share a post/page with others. Supports e-mail and posting to social bookmarking sites. Thanks to Thomas McMahon for footwork on the URLs. Questions on configuration, etc.? Make sure to read the README.
    [ Autor: Alex King ] [ url ]
  • Shashin 2.1
    A plugin for integrating Picasa photos in WordPress.
    [ Autor: Michael Toppa ] [ url ]
  • Simple Tags 1.5.7
    Simple Tags : Extended Tagging for WordPress 2.3 and 2.5 ! Autocompletion, Suggested Tags, Tag Cloud Widgets, Related Posts, Mass edit tags !
    [ Autor: Amaury BALMER ] [ url ]
  • WordPress.com Stats 1.2.1
    Tracks views, post/page views, referrers, and clicks. Requires a WordPress.com API key.
    [ Autor: Andy Skelton ] [ url ]
  • WP-Footnotes 2.2
    Allows a user to easily add footnotes to a post.
    [ Autor: Simon Elvery ] [ url ]
  • WP-PluginsUsed 1.90
    Display WordPress plugins that you currently have (both active and inactive) onto a post/page.
    [ Autor: Lester 'GaMerZ' Chan; Mario Schubert ] [ url ]
  • WP-PostViews 1.20
    Enables you to display how many times a post/page had been viewed. It will not count registered member views, but that can be changed easily.
    [ Autor: Lester 'GaMerZ' Chan ] [ url ]

Inactive Plugins

  • Exec-PHP 4.7
    Executes <?php ?> code in your posts, pages and text widgets.
    [ Autor: Sören Weber ] [ url ]

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]]> http://en.doktor-schubert.net/2007/used-plugins/feed/ Our Discovery Hits the News http://en.doktor-schubert.net/2007/our-discovery-hits-the-news/ http://en.doktor-schubert.net/2007/our-discovery-hits-the-news/#comments Mon, 06 Aug 2007 10:55:23 +0000 Mario http://www.doktor-schubert.net/uncategorized/unsere-entdeckung-ist-eine-newsmeldung-wert/ The German Rhombos Nachrichtenservice wrote:

Trojanereinsatz gegen Bauchspeicheldrüsenkrebs Stammzellen spielen eine Rolle beim Wachstum von Geschwülsten und könnten in modifizierter Form einen therapeutischen Ansatz bieten.

The complete text of the newsflash can be found at
http://www.rhombos.de/shop/a/show/story/?1155.

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]]> http://en.doktor-schubert.net/2007/our-discovery-hits-the-news/feed/ EPC Award for Best Basic Science Paper http://en.doktor-schubert.net/2007/epc-best-basic-science-paper/ http://en.doktor-schubert.net/2007/epc-best-basic-science-paper/#comments Fri, 20 Jul 2007 18:28:08 +0000 Mario http://www.doktor-schubert.net/uncategorized/epc-auszeichnung-als-bestes-grundlagenpaper/ EPC Award 2007 for best basic science paperMeine Kooperation mit der Arbeitsgruppe von Prof. Ingrid Herr (Molekulare Onkochirurgie) und PD. Peter Büchler (Viszeralchirurgie) über die Rolle von mesenchymalen Stammzellen bei der Entstehung und der Therapie des duktalen Pankreaskarzinoms trägt erste Früchte: Beim diesjährigen 39. Treffen des European Pancreatic Club (EPC) in Newcastle, UK erhielt unsere Publikation “BONE MARROW DERIVED MESENCHYMAL STEM CELLS FOR THERAPY OF PANCREATIC CANCER” den Preis als bestes Grundlagenpaper (”EPC 2007 Award for best basic science paper”). Mein Glückwunsch und Dankeschön für die gute Zusammenarbeit gilt insbesondere meinen Koautoren aus der AG Herr.

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]]> http://en.doktor-schubert.net/2007/epc-best-basic-science-paper/feed/ Keystone-Meeting 2007 http://en.doktor-schubert.net/2007/keystone-meeting-2007/ http://en.doktor-schubert.net/2007/keystone-meeting-2007/#comments Sun, 11 Mar 2007 16:10:10 +0000 Mario http://www.doktor-schubert.net/uncategorized/keystone-meeting-2007/ Vom 03. bis 08. März 2007 hatte ich die Gelegenheit das jährliche Stammzellmeeting in Keystone, Colorado zu besuchen. Vier Tage lang gab es exzellente Vorträge, Seminare und Postersessions rund um die Themen normale und leukämische Stammzellen und Stammzellnische.

Bilder aus Keystone

Blick aus meinem Hotelzimmer, 6:15 Uhr Ortszeit
Rocky Mountains, Summit Region @ 6:15 Ortszeit
Mein Hotel, das Keystone Inn
Dallas Airport
Airbus A360-600 @ Dallas Airport
The Great Plains near Dallas, Colorado.
London, Luftfoto.
Eisskulptur am Keystone Lodge

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]]> http://en.doktor-schubert.net/2007/keystone-meeting-2007/feed/ First script submitted to the DGCH http://en.doktor-schubert.net/2005/first-script-submitted-to-the-dgch/ http://en.doktor-schubert.net/2005/first-script-submitted-to-the-dgch/#comments Sun, 16 Jan 2005 16:58:10 +0000 Mario http://www.doktor-schubert.net/uncategorized/first-script-submitted-to-the-dgch/ Third Thought: ArchivbeitragI’ll be speaking at the annual meeting of the german surgical society (DGCH) which takes place in munich from April, 5 to April, 8. During the “surgical forum” i will be giving a lecture about the role of notch-signalling for the neurovascular developement in pancreas carcinomas. the script will then be published to the surgical DGCH-yearbook, which, unfortunately, isn’t citated by medline.

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