Research

semi-automated translation Since the year 2000 – first as a medical student, now as a principal investigator – I am researching in the field of blood stem cells for the development of new therapeutic approaches for cancer – especially with respect to acute and chronic leukemia (blood cancer).
On this page you will find more information about my current research projects, and my Publications.

What You Can Do

There are many ways that you can support my research:
- By donating money which would allow it to purchase urgently needed new equipment (refrigerated centrifuge, Western blot chamber, microscope).
- By donating items. Currently, we need a computer, printer and fax machine, and a refrigerator.
- Through a bone marrow donation, which we would us as a comparison test for various research projects.

My Research Laboratory

As a research associate in the group of Prof. Anthony D. Ho since 2006, I run my own small research laboratory currently with five employees (two medical doctoral students, two Diplombiologinnen and assigned me a medical technician). Apart from two lab rooms with the usual equipment for cellular and molecular biological research, I have a laboratory in the security area (S2), and the conditions necessary for the work with (lenti) viral vectors.

Used methods

In my lab, or in the working group established in Ho regularly methods used are:

Processing of bone marrow, peripheral blood and mobilisiertem umbilical cord blood to isolate hematopoietic stem cells.
Magnetic cell separation (MACS) and FACS-Sortierung/-Messung.
Elaborate cell culture assays, such as CFU-GEMM, LTC-IC, NK-ML-IC and IC.
Semiquantitative (RT) PCR, quantitative real-time PCR (TaqMan ®).
Protein determination using Western blot.
Vector cloning, transformation competent bacteria, CaCl₂-transfection.
Lentivirale transduction (eGFP-marker; Gene silencing using shRNA).